Neurotensin terminals form synapses primarily with neurons lacking detectable tyrosine hydroxylase immunoreactivity in the rat substantia nigra and ventral tegmental area
Identifieur interne : 004560 ( Main/Exploration ); précédent : 004559; suivant : 004561Neurotensin terminals form synapses primarily with neurons lacking detectable tyrosine hydroxylase immunoreactivity in the rat substantia nigra and ventral tegmental area
Auteurs : John Woulfe [Canada] ; Alain Beaudet [Canada]Source :
- Journal of Comparative Neurology [ 0021-9967 ] ; 1992-07-01.
English descriptors
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Abstract
A light and electron microscopic double antigen localization technique was employed to examine the fine structural relationship between neurotensin‐containing axon terminals and dopaminergic neurons in the substantia nigra and ventral tegmental area of the rat. At the light microscopic level, neurotensin‐immunoreactive terminals were densely distributed throughout the substantia nigra pars compacta and ventral tegmental area in close proximity to tyrosine hydroxylase‐immunoreactive somata and dendrites. On electron microscopic examination, direct synaptic connections were identified between neurotensin‐immunoreactive axon terminals and tyrosine hydroxylase‐immunopositive perikarya and dendrites. However, only 8.2% and 8.8% of the neurotensin‐immunoreactive axonal profiles detected in the substantia nigra and ventral tegmental area, respectively, were found in direct apposition with tyrosine hydroxylase‐immunostained elements. In turn, only 9.3% and 10.0% of tyrosine hydroxylase immunoreactive dendrites sampled from the substantia nigra and ventral tegmental area, respectively, were seen in contact with neurotensin immunopositive axon terminals. However, neurotensin‐immunoreactive and tyrosine hydroxylase‐immunolabelled elements were frequently identified in close anatomical proximity (<5 μm) to one another. These results are interpreted in light of the selective association of neurotensin receptors with dopaminergic neurons in the substantia nigra and ventral tegmental area to suggest a predominantly parasynaptic mechanism of action for neurotensin in the ventral midbrain.
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DOI: 10.1002/cne.903210114
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Neurotensin terminals form synapses primarily with neurons lacking detectable tyrosine hydroxylase immunoreactivity in the rat substantia nigra and ventral tegmental area</title><author><name sortKey="Woulfe, John" sort="Woulfe, John" uniqKey="Woulfe J" first="John" last="Woulfe">John Woulfe</name></author><author><name sortKey="Beaudet, Alain" sort="Beaudet, Alain" uniqKey="Beaudet A" first="Alain" last="Beaudet">Alain Beaudet</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:CBBEE9F3C6911BFCAD13AE88D835BE1796621868</idno><date when="1992" year="1992">1992</date><idno type="doi">10.1002/cne.903210114</idno><idno type="url">https://api-v5.istex.fr/document/CBBEE9F3C6911BFCAD13AE88D835BE1796621868/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">002377</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">002377</idno><idno type="wicri:Area/Istex/Curation">002377</idno><idno type="wicri:Area/Istex/Checkpoint">001E08</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001E08</idno><idno type="wicri:doubleKey">0021-9967:1992:Woulfe J:neurotensin:terminals:form</idno><idno type="wicri:Area/Main/Merge">004E10</idno><idno type="wicri:Area/Main/Curation">004560</idno><idno type="wicri:Area/Main/Exploration">004560</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Neurotensin terminals form synapses primarily with neurons lacking detectable tyrosine hydroxylase immunoreactivity in the rat substantia nigra and ventral tegmental area</title><author><name sortKey="Woulfe, John" sort="Woulfe, John" uniqKey="Woulfe J" first="John" last="Woulfe">John Woulfe</name><affiliation wicri:level="4"><country xml:lang="fr">Canada</country><wicri:regionArea>Montreal Neurological Institute, McGill University, Montreal, Quebec, H3A 2B4</wicri:regionArea><orgName type="university">Université McGill</orgName><placeName><settlement type="city">Montréal</settlement><region type="state">Québec</region></placeName></affiliation></author><author><name sortKey="Beaudet, Alain" sort="Beaudet, Alain" uniqKey="Beaudet A" first="Alain" last="Beaudet">Alain Beaudet</name><affiliation wicri:level="4"><country xml:lang="fr">Canada</country><wicri:regionArea>Montreal Neurological Institute, McGill University, Montreal, Quebec, H3A 2B4</wicri:regionArea><orgName type="university">Université McGill</orgName><placeName><settlement type="city">Montréal</settlement><region type="state">Québec</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Comparative Neurology</title><title level="j" type="abbrev">J. Comp. Neurol.</title><idno type="ISSN">0021-9967</idno><idno type="eISSN">1096-9861</idno><imprint><publisher>Wiley‐Liss, Inc.</publisher><pubPlace>New York</pubPlace><date type="published" when="1992-07-01">1992-07-01</date><biblScope unit="volume">321</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="163">163</biblScope><biblScope unit="page" to="176">176</biblScope></imprint><idno type="ISSN">0021-9967</idno></series><idno type="istex">CBBEE9F3C6911BFCAD13AE88D835BE1796621868</idno><idno type="DOI">10.1002/cne.903210114</idno><idno type="ArticleID">CNE903210114</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0021-9967</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>electron microscopy</term><term>immunocytochemistry</term><term>neuropeptides</term><term>paracrine transmission</term><term>tyrosine hydroxylase</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A light and electron microscopic double antigen localization technique was employed to examine the fine structural relationship between neurotensin‐containing axon terminals and dopaminergic neurons in the substantia nigra and ventral tegmental area of the rat. At the light microscopic level, neurotensin‐immunoreactive terminals were densely distributed throughout the substantia nigra pars compacta and ventral tegmental area in close proximity to tyrosine hydroxylase‐immunoreactive somata and dendrites. On electron microscopic examination, direct synaptic connections were identified between neurotensin‐immunoreactive axon terminals and tyrosine hydroxylase‐immunopositive perikarya and dendrites. However, only 8.2% and 8.8% of the neurotensin‐immunoreactive axonal profiles detected in the substantia nigra and ventral tegmental area, respectively, were found in direct apposition with tyrosine hydroxylase‐immunostained elements. In turn, only 9.3% and 10.0% of tyrosine hydroxylase immunoreactive dendrites sampled from the substantia nigra and ventral tegmental area, respectively, were seen in contact with neurotensin immunopositive axon terminals. However, neurotensin‐immunoreactive and tyrosine hydroxylase‐immunolabelled elements were frequently identified in close anatomical proximity (<5 μm) to one another. These results are interpreted in light of the selective association of neurotensin receptors with dopaminergic neurons in the substantia nigra and ventral tegmental area to suggest a predominantly parasynaptic mechanism of action for neurotensin in the ventral midbrain.</div></front></TEI><affiliations><list><country><li>Canada</li></country><region><li>Québec</li></region><settlement><li>Montréal</li></settlement><orgName><li>Université McGill</li></orgName></list><tree><country name="Canada"><region name="Québec"><name sortKey="Woulfe, John" sort="Woulfe, John" uniqKey="Woulfe J" first="John" last="Woulfe">John Woulfe</name></region><name sortKey="Beaudet, Alain" sort="Beaudet, Alain" uniqKey="Beaudet A" first="Alain" last="Beaudet">Alain Beaudet</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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